Download The Protein Protocols Handbook by John M. Walker PDF

By John M. Walker

Univ. of Hertfordshire, Hatfield, united kingdom. offers a cross-section of analytical thoughts favourite for proteins and peptides. each one bankruptcy opens with an outline of the elemental conception in the back of the tactic being defined. define layout. prior version: c1996. Hardcover, softcover additionally to be had.

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1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248–254. 2. Chial, H. , Thompson, H. , and Splittgerber, A. G. (1993) A spectral study of the charge forms of Coomassie Blue G. Analyt. Biochem. 209, 258–266. 3. Compton, S. J. and Jones, C. G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Analyt. Biochem. 151, 369–374. 4. Congdon, R. , Muth, G. , and Splittgerber, A.

1981) Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Analyt. Biochem. 116, 53–64. 7. Stoscheck, C. M. (1990) Increased uniformity in the response of the Coomassie Blue protein assay to different proteins. Analyt. Biochem. 184, 111–116. 8. Spector, T. (1978) Refinement of the Coomassie Blue method of protein quantitation. 5 to 50 µg of protein. Analyt. Biochem. 86, 142–146. 9. Pande, S. V. and Murthy, M. S. R. (1994) A modified micro-Bradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents, and lipids.

216, 232–233. BCA for Protein Quantitation 11 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation John M. Walker 1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter 2). The Cu+ is then detected by reaction with BCA. The two assays are of similar sensitivity, but since BCA is stable under alkali conditions, this assay has the advantage that it can be carried out as a one-step process compared to the two steps needed in the Lowry assay.

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