By Amersham Biosciences
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Additional resources for Reversed Phase Chromatography
However, to minimise the risk of losing unbound material in the flow-through fractions or losing resolution, lower and more practical loading ranges are recommended. The detection limit for one peak may be below 1 ng under optimal conditions. 38 Availability Both columns are designed specifically for use with SMART System. They can be connected to ÄKTApurifier or other high performance chromatography systems via a Precision Column Holder (Code No. 17-1455-01). Ordering information is shown in Section 7.
D. 01 - 500 Table 4. Chemical and Physical Stability µRPC C2/C18 can be used with aqueous and organic solvents miscible in water in the pH range 2 - 8. As with all silica based media extended exposure to pH extremes should be avoided as the matrix begins to degrade at pH values greater than 7 - 8 and less than 2 - 3. 0. Additives such as guanidine hydrochloride, urea, formic acid (< 60%) and detergents may be used with µRPC C2/C18. Both columns may be operated over the temperature range of 4 - 40 °C up to the maximum pressures shown in Table 4.
Note the uniform size distribution. 5 µm The pore size distribution, batch-to-batch reproducibility (Fig. 20) and excellent scalability (Fig 21) of SOURCE RPC ensure outstanding chromatographic properties at any scale of operation. 30 Fig. 20. Reproducibility of three production batches of SOURCE 15RPC. 5 mg/ml) 25 µl applied. d. 1% TFA, 60% acetonitrile in water 15-65% B in 20 min 1 ml/min Column: Eluent A: Eluent B: Gradient: Flow: Batch 1 Batch 2 Retention time (min) 13 Batch 3 12 11 10 9 8 IIe7 Angio III Fig.