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1). To determine the isomeric distribution of allylic hydroxyooctadecenoate derivatives, cis and trans fractions were separated by silver nitrate-thin layer chromatography (TLC), a procedure that separates according to the number, position and geometry of double bonds, and they were hydrogenated prior to GC-MS analyses of the TMS ether derivatives. 1). These hydroperoxide isomers were better separated as the hydroxy octadecenoate derivatives by the same silica HPLC method and re-analysed by GC-MS.

4–62 (1973) (edited by J. K. , New York. U. W. A. O. , Westport, CT. E. Initiation of lipid peroxidation in biological systems. CRC Critical Reviews Food Science and Nutrition 25, 317–364 (1987). P. Kinetics of lipid oxidation in foods. CRC Food Technol. 2, 355–405 (1971). O. P. Accelerated shelf-life testing for oxidative rancidity in foods – a review. Food Chem. 2, 291–308 (1977). A. Pathways in autoxidation, in Peroxide Reaction Mechanisms, pp. 107–128 (1962) (edited by J. O. , New York. M. Metals and lipid oxidation.

Further knowledge acquired on the structures of hydroperoxides led to important advances in clarifying the structures of their decomposition products that contribute to the problems of rancidity in foods, and to free radical damage in biological systems. Organic structural studies of oxidation products obtained with lipids relevant to foods and biological systems are thus more useful than kinetic studies relying on artificial initiators, and simplistic models carried out under improbable reaction conditions.

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