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By Christopher G Herbert; R A W Johnstone

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Adopting a method of Porâth et al (1983) and Fanou-Ayi and Vijayalakshmi (1983), El Rassi and Horvâth (1986) coupled IDA ligands to an epoxy-derivatized silica and used it in the Cu2+ complexated form. G. Bonded Silicas for Size Exclusion Chromatography Size exclusion chromatography (SEC) of biopolymers introduced by Porâth and Flodin (1959) was traditionally performed on soft organic gels with aqueous buffered eluents in a technique known as gel filtration chromatography. Porous glasses were first employed as inorganic supports (CPG, 1981).

Silica-Based Size Exclusion Packings Commercial size exclusion columns are marketed in various pore sizes ranging from 6 to 400 nm (see Table Vili) (Unger and Kinkel, 1988). Columns have lengths up to 250-300 mm and inner diameters between 4 and 10 mm. Most of the SEC packings have particle sizes in the 10-μηι size range except the two DuPont Zorbax Bio Series Gel Filtration columns GF 250 (dp = 4 - 5 μπι) and GF 450 (dp % 6 μιη). In order to improve the pH stability of these two TABLE VII Commercial Silica-Bonded Affinity Packings Name Supplier Bakerbond MA b Bakerbond ABX Baker Baker Bakerbond Ultraprep Affinity Matrices (activated silica supports) Affinity Matrices (activated silica supports) Affinity Matrices (activated silica supports) Ultraffinity-EP CPG/CDI-Activated Glycophase CPG/Aminoacryl CPG/Aminopropyl CPG/Carboxyl CPG/Long-Chain Alkylamine CPG/NHS Glycophase CPG/Stable Diazonium Salt CPG/Thiol Baker Baker Baker Baker Baker Beckman Pierce Pierce Pierce Pierce Pierce Pierce Pierce Pierce a Form 0 Mean particle diameter dp (μιτι) Nominal pore size PA (nm) o, x o, x 5,15,40 5,15,40 28,30 28,30 X 40 40 40 40 40 10 74-125 125-177 125-177 125-177 125-177 125-177 125-177 125-177 28 28 28 28 28 — X X X — x,b x,b x,b x,b x,b x,b x,b x,b Abbreviations: o, spherical particles; b, bulk material; x, irregular particles; —, not given.

Box DH 310, Lafayette, IN 47902 TOSOH Ltd. , 9 Bridgewell Place, Clifton, NJ 07014 60 Κ. Κ. Unger et al. column types, the silica was surface treated with zirconia before modification (Stout and DeStefano, 1985). As far as detailed information is given, most of the silica-bonded phases for SEC have a glycerolpropyl functionality, either linked as a monolayer or as a multilayer. The most popular columns are the TSK SW series. The parent silica is a highly porous material loaded with a hydrophilic polymer layer that almost completely covers the original silica surface.

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