By Perry G. Wang, Weixuan He
Content material: Aqueous normal-phase chromatography: the bridge among reversed-phase and HILIC / Joseph J. Pesek, Maria T. Matyska -- program of the calculated physicochemical estate, log D, to hydrophilic interplay chromatography within the bioanalytical laboratory / Eugene P. Kadar, Chad E. Wujcik, Olga Kavetskaia -- HILIC habit of reversed-phase/ion-exchange mixed-mode desk bound levels and their purposes / Xiaodong Liu, Christopher A. Pohl -- Mechanisms controlling the separation and retention of proanthocyanidins and different plant polyphenols in hydrophilic interplay chromatography / Akio Yanagida, Yoichi Shibusawa -- purposes of hydrophilic interplay chromatography to nutrition research / Leticia Mora ... [et al.] -- Hydrophilic interplay liquid chromatography-mass spectrometry (HILIC-MS) of paralytic shellfish poisoning pollutants, domoic acid, and diverse cyanobacterial pollution / Carmela Dell'Aversano -- the applying of HILIC for polar environmental contaminants (including prescription drugs) in aquatic structures / Alexander L.N. van Nuijs ... [et al.] -- Hydrophilic interplay chromatography-mass spectrometry: an environmental program / Kerry M. Peru ... [et al.] -- medical purposes of hydrophilic interplay liquid chromatography / Ping Wang -- Dimethylarginine research by means of HILIC-MS/ MS and its functions in scientific chemistry and in vivo animal types / O. D'Apolito ... [et al.] -- Polar practical teams for HILIC procedure / Zhigang Hao -- research of pharmaceutical impurities utilizing hydrophilic interplay liquid chromatography / Mingjiang solar, David Q. Liu -- quick in-process strategy for the choice of Ioversol and similar polar compounds through hydrophilic interplay chromatography (HILIC) and UPLC / Yuming Chen ... [et al.] -- Retention habit of hydrazine derivatives in HILIC mode / Juris Hmelnickis, Igors Susinskis, Kaspars Kokums -- HILIC retention habit and process improvement for hugely polar easy compounds utilized in pharmaceutical synthesis / Minhui Ma, Min Liu -- Retention of polar acidic and simple medicines through HILIC / Mohammed Shahid Ali, Aamer Roshanali Khatri -- Retention and selectivity of polar desk bound levels for hydrophilic interplay chromatography / Yong Guo -- HILIC-MS/MS for the selection of polar bioactive ingredients: consultant functions within the fields of pharmacokinetics and metabolic profiling / Yannis L. Loukas, Yannis Dotsikas -- procedure improvement and research of mono- and diphosphorylated nucleotides by way of HILIC HPLC-ESI-MS / Samuel H. Yang, Hien P. Nguyen, Kevin A. Schug -- Hydrophilic interplay liquid chromatography-based enrichment protocol coupled to mass spectrometry for glycoproteome research / Cosima Damiana Calvano -- improvement and alertness of tools for separation of carbohydrates by means of hydrophilic interplay liquid chromatography / Goran Karlsson -- Hydrophilic interplay liquid chromatography within the characterization of glycoproteins / Joanne E. Nettleship -- research of protein glycosylation and phosphorylation utilizing HILIC-MS / Morten Thaysen-Andersen, Kasper Engholm-Keller, Peter Roepstorff
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Additional info for Hydrophilic interaction liquid chromatography (HILIC) and advanced applications
1. Thus, the longer retention for fumaric acid most likely is the result of two ionized sites at the mobile phase pH while maleic acid only has one of its acidic groups ionized under these conditions. 14. The overlaid EICs in the negative ion mode are for three m/z values taken from several donor saliva samples. 22 The inset shows expanded EICs for adipic acid. 5 0 8 9 10 11 12 13 Counts vs. acquisition time (min) 3 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Counts vs. 14 EICs for organic acids from saliva samples on the DH column.
1 mm × 150 mm. 3 μg/mL. J. , J. Chromatogr. 1 4 0 1 2 3 4 5 6 7 8 9 10 11 12 Counts (%) vs. 13 EICs of organic acids on a DH column. Detection in negative ion mode. Maleic acid (1), m/z = 115; aconitic acid (2,3,4), m/z = 173; fumaric acid (5), m/z = 115; citric acid (6), m/z = 191. 1% ammonium formate. 0 min 30% B. J. , J. Chromatogr. 23 Since the organic acids that have been identified as metabolites can contain one, two, or three acidic functional groups, this feature alone provides for significant differences in chemical properties that can be utilized to control their retention.
29. , Proc. Natl. Acad. Sci. USA, 2004, 101, 11239. 30. I. , Biosci. , 2002, 22, 501. 31. , Vitam. , 2001, 61, 103. 32. H. , Crit. Rev. Clin. Lab. , 1999, 36, 275. 33. , Adv. Exp. Med. , 1997, 426, 73. 34. , J. Sep. , 2007, 30, 1150. © 2011 by Taylor and Francis Group, LLC of 2 Application the Calculated Physicochemical Property, Log D, to Hydrophilic Interaction Chromatography in the Bioanalytical Laboratory Eugene P. Kadar, Chad E. 4 Introduction.....................................................................................................