Download Enzyme Structure Part I by Nathan P. Kaplan, Nathan P. Colowick, C. H.W. Hirs, Serge N. PDF

By Nathan P. Kaplan, Nathan P. Colowick, C. H.W. Hirs, Serge N. Timasheff

The seriously acclaimed laboratory normal, Methods in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable at the present time - actually a vital e-book for researchers in all fields of lifestyles sciences

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Exchange resin, buffers, etc. that are commonly used with the amino acid a n a l y z e r , ar A s i n g l e - c o l u m n a n a l y s i s is u s u a l l y m a d e s o t h a t a l l y i e l d s m a y be calculated from the one loading11; also it ensures that amino acids eluted after phenylalanine and before the basic amino acids are detected. T h e e l u t i o n b e h a v i o r o f P E - c y s t e i n e is s e n s i t i v e t o p H a n d t e m p e r a t u r e , CySS01~ AM o so t~ 150 ioo 2OO 2~0 TIME Fso.

Fontana, Int. J. Pept. Protein Res. 15, 102 (1980). 9 A. Mondino and G. Bongiovanni, J. Chromatogr. 52, 405 (1970). 10 R. J. Simpson, M. R. Neuberger, and T. Y. Liu, J. Biol. Chem. 251, 1936 (1976). 11 A. S. Inglis, D. T. W. McMahon, C. M. Roxburgh, and H. Takayanagi,Anal. Bioehem. 72, 86 (1976). 28 AMINOACIDANALYSIS [5] procedure. Iflysozyme is used, since it contains only disulfide groups and no sulfhydryl groups, the alkylation is carded out in the presence of mercaptoethanol (100-molar excess over total disulfides) using a 3-fold excess of 4-vinylpyridine over total sulfhydryl groups.

Biochem. 7, 495-509 (1977). 11 j. K. W. Mardian and I. Isenberg, Anal. Biochem. 91, 1-12 (1978). METHODS IN ENZYMOLOGY,VOL. 91 Copyrighl © 1983 by Academic Press, Inc. All rights of reproduction in any formreserved. ISBN 0-12-181991-4 [6] AMINO ACID COMPOSITION OF PROTEINS 37 "background," and to illustrate, with a standard protein, the type of results obtainable in a clean experiment. TM No attempt will be made to define the best procedure for running gels la-16 or analyzing material from the gel slices4-1° nor to compare elution procedures with direct gel analysis.

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