By P. VINCENZINI and R. BARBUCCI
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Extra info for Biomedical Applications of Nano Technologies: Advances in Science and Technology
40,41 The approach discussed in this letter relies on adding a gel structure to a preexisting membrane, rather than forming a membrane on a pre-cast gel. 42,43 Their technique, however, has not been shown to achieve sufficient membrane resistances for single-molecule measurements. Our approach maintains high membrane resistance by photopolymerizing the gel in situ around a high-quality membrane. Materials and methods Freestanding microchannel fabrication PDMS microchannels were fabricated by molding a liquid 10:1 PDMS base:crosslinker mixture (Dow Corning) around 200 µmdiameter silver wires and curing at 60 °C for 2 hours.
Y. "Synthesis studies of sputtering TiO2 films on poly(dimethylsiloxane) for surface modification" Colloid Surface A 272: 170-175 (2006). ; Roberts, R. ; Arnold, F. ; Quake, S. R. "Dynamic pattern formation in a vesicle-generating microfluidic device" Phys Rev Lett 86(18): 4163-4166 (2001). ; Roach, L. ; Ismagilov, R. F. "Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets" J Am Chem Soc 125(37): 11170-11171 (2003). ; Ismagilov, R. F. "Millisecond kinetics on a microfluidic chip using nanoliters of reagents" J Am Chem Soc 125(47): 14613-14619 (2003).
49 Combining these two techniques will allow for the facile, automated formation of very long-lived lipid bilayers: a capacity that will be valuable in both high-throughput screening and sensing applications. Conclusions There are many potential applications of membrane channel proteins in lipid bilayers; practical implementation of these applications, however, requires new methods for dealing with lipid bilayers, which are notoriously fragile and difficult to fabricate. We have developed two techniques which fill this demand.