Download Advances in Lipid Methodology. Volume 3 by W. W. Christie PDF

By W. W. Christie

This is often the 3rd quantity of an occasional sequence of assessment volumes facing features of lipid method. As with the 1st volumes, issues were chosen which were constructing speedily lately and feature a few significance to lipid research. The authors are all top overseas experts.

Topics lined contain: research of positional isomers of glycerolipids by way of non-enzymatic tools, separation of phospholipid sessions by way of high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid part behaviour and lipid diffusion, between others

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Because urine and other body fluids are water solutions containing large quantities of dissolved solutes and cells, the phospholipid extraction procedure must be modified from that used for tissue phospholipids [11 ]. 2 mm polysulfone membrane filter to remove cells and cellular fragments. 2 M, and the pH adjusted to 6. Chloroform/methanol (2:1) is then added to this EDTA-treated urine filtrate in a ratio of 5:1 (CHCl3MeOH/H20), shaken vigorously, and the mixture subsequently worked up for NMR analysis.

The sn-1-enantiomers were eluted ahead of the corresponding sn-3-enantiomers. Complete separation of the sn-2-isomers from the corresponding enantiomers and partial separation of the enantiomer homologues differing by two acyl carbons was also observed. The sn-2-isomer was eluted ahead of the sn-1-isomer. g. sn-1-12:0 and sn-3-18:0. Such critical pairs could not be separated on the column under the conditions used. The separation of homologues differing by two carbons was poor compared to the enantiomer separation.

Christie, Oily Press, Ayr, Scotland). 15. W. , Biochim. Biophys. Acta, 176, 445-452 (1969). 16. , Laakso,P. , J. Am. Oil Chem. , 68, 695-701 (1991). 17. J. , Lipids, 28, 217-222 (1993). 18. , Rev. Fr. Corps Gras, 40, 40-47 (1993). 19. S. ,Anal. , 127, 37-48 (1982). 20. , Malinski,E. , lipids, 21, 434439 (1986). 21. D. , lipids, 22, 362-365 (1987). 22. A. , Lipids, 26, 769-773 (1991). 23. E. , Lipids, 25, 40-47 (1990). 24. , Ohlson,R. , Chem. Phys. lipids, 14, 174-188 (1975). 25. , in Advances in Lipid Methodology - Two, pp.

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